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KMID : 0369820050350020075
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Jorunal of Korean Pharmaceutical Sciences
2005 Volume.35 No. 2 p.75 ~ p.80
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Lactic Acid Bacteria as Oral Antigen Protein Carriers
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조희정/Cho HJ
최한곤/김정애/오유경/Choi HG/Kim JA/Oh YK
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Abstract
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A promising application of Lactococcus lactis is its use as live vehicles for production and delivery of heterologous proteins of vaccines and therapeutic substances. Because L. lactis has GRAS ('generally regarded as safe') status, we tested whether L. lactis could function as the carrier of the Ll protein of human papillomavirus (HPV) type 16. The RNA level expression of Ll gene was detected in L. Lactis. The Ll protein was expressed in L. lactis with Ll gene. The growth of strains L. lactis with an empty plasmid (pAMJ328) and L. lactis with Ll-encoding plasmid (pAMJ328-Ll) was slightly decreased in comparison with the growth of strains L. lactis (wild type). However, all the three strains of L. lactis maintained the ability to ferment sugars primarily into lactic acid, indicating that Ll protein did not affect the biochemical property of L. lactis. These results suggest that L. lactis, capable of carrying Ll protein, might be further developed as a biocompatible oral protein delivery system.
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KEYWORD
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Lactococcous Lactis, oral carrier, protein delivery system
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